Toma E. Tomov

Computer Controlled DNA Based Molecular Machine

Seminar Room ZNN, 2nd Floor

08.10.2015, 14:00

The manufacturing of well-functioning machines is a major challenge of modern technology and science. DNA nanotechnology is probably the most promising path towards realization of this goal on the molecular level. It was demonstrated that DNA based devices and motors could potentially be used in a variety of applications. However, despite variety of architectures and operation principles, devices and particularly motors, still suffer from slow and incomplete stepping reactions along with harmful side products. For the technology to be applicable, it is essential that the devices will operate as commanded, with high operating efficiency and speed, while maintaining structural integrity.

Wishing to understand the reasons for these unwanted reactions and offer solutions, we used a non-autonomous bipedal DNA motor. Upon sequential introduction of DNA fuels and anti-fuels, the motor strides on a DNA track which is embedded on a DNA-origami. We utilize single-molecule FRET/ALEX and TIRF spectroscopy along with computer controlled microfluidics device to study the structure, interaction, kinetic and yield. 

We demonstrate that careful analysis of motor kinetics promotes rational design that significantly can improve motor operation over unprecedented distance, processivity and speed. The motor preforms 36 consecutive steps, crossing a distance of 400 nm, with 50% overall yield, which is equivalent to more than 99% yield per chemical reaction, and the stepping rate can be as fast as several seconds per step.

This novel combination of DNA nanotechnology, microfluidics technology and single-molecule fluorescence spectroscopy holds a great promise for future computer control molecular machines.