Eugen Kerkhoff

Dissection of Spir function: actin nucleation versus motor protein targeting

PH127

30.05.2014, 13:00


Janine Tittel1*, Tobias Welz2*, Susanne Dietrich2, Annette Samol-Wolf2, Sabine Weiss2, Andreas Grasskamp2, Thomas Weidemann1, Petra Schwille1, Martin Kollmar3 and Eugen Kerkhoff2

1Max Planck Institute of Biochemistry, Martinsried; 2University Hospital Regensburg, Regensburg; 3Max Planck Institute for Biophysical Chemistry, Göttingen; *equal contribution

 

Spir-1, -2 actin nucleators and formin-2 cooperatively initiate actin filaments at vesicle membranes. In mouse metaphase oocytes actin filaments function as tracks for long-range transport of Rab11 vesicles towards the oocyte cortex. The vesicle motility is mediated by the myosin Vb actin motor protein, which is an effector of the Rab11 small G protein. Here we uncover an intricate link between actin nucleation and the engagement of motor proteins. In model membrane systems, vesicle targeting depends on  the Spir-2 FYVE-type zinc finger membrane interaction domain and negatively charges lipids. In a cellular context additional protein-protein interactions are required, as both the knock down of Rab11 and treatment with brefeldin A, a Arf small G protein inhibitor, releases Spir proteins from intracellular membranes into the bulk cytoplasm. We detected a direct interaction between Spir-2 and Arf1 but not between Spir-2 and Rab11. Instead we could immunoprecipitate a tripartite complex of Spir-2, myosin Vb and Rab11. Pairwise expression of wild-type and mutant Spir-2 and myosin Vb proteins revealed that each of them can recruit mutants that would not associate with vesicle membranes on their own. In addition, using a bioinformatics approach, we identified a conserved, yet unrecognized sequence in Spir that is crucial for the interaction with the myosin Vb globular tail domain. Thus, the Spir/myosin interaction plays an important role for the assembly of a stable actin nucleator/motor protein complex at vesicle membranes.