Achillefs Kapanidis, University of Oxford, U.K.

Single-Molecule Fluorescence Studies of Biomolecular Dynamics

PH 127

28.01.2011, 13:00


Abstract:

Single-molecule fluorescence methods uncover the presence of static and dynamic heterogeneity in many biomolecular systems. I will discuss the use of single-molecule Foerster resonance energy transfer in studies of the conformational dynamics of DNA polymerase and derivatives thereof with decreased fidelity for DNA synthesis. I will also discuss two new single-molecule methods: switchable-FRET, a method that allow measurements of multiple distances within a single molecule by combining FRET with acceptor photo switching; and DAO-STORM, a new algorithm that enables super-resolution imaging in crowded field of molecules.